Ozone postconditioning in renal ischaemia-reperfusion model. Functional and morphological evidences

Ischaemia-reperfusion is one of the main causes of kidney complications. The most frequent lesion is acute tubular necrosis.

Ozone oxidative preconditioning exerts a modulatory effect of redox state of renal cells in models of ischaemia-reperfusion, by stimulating endogenous antioxidant mechanisms. Similar results have been obtained in more recent studies using ischaemic postconditioning.

Objectives: To evaluate the effect of ozone oxidative postconditioning on renal function and morphology in an ischaemia-reperfusion rat model. Methods: We used forty female Wistar rats weighing between 150g-200g randomly divided into 4 groups (negative control, positive control, oxygen and ozone).

The groups: positive control, oxygen and ozone were subjected to 60 minutes of ischaemia and 10 days of reperfusion.

During reperfusion, the oxygen group was given 26mg/kg body weight of oxygen, and the ozone group 0.5mg/kg body weight of ozone, rectally. At the end of the experiment urine and blood samples were taken for renal function tests and kidneys were removed for histological study.

Results: The ozone group showed no significant differences for filtration fraction and proteinuria compared to the negative control group. The glomerular filtrate rate, renal plasma flow and creatinine showed a slight improvement in comparison with oxygen and positive control groups.

The ozone group showed significantly less overall histological damage than the positive control and oxygen groups. Conclusions: Ozone postconditioning showed to have a protective effect in preserving renal function and morphology.

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