Master’s Dissertation in Bioengineering by Tatiane Costa Quaresma
Institute of Biomedical Engineering, Camilo Castelo Branco University – São José dos Campos – 2013

Hands are the main route of transmission of microorganisms and possible new cases of nosocomial infection. The practice of hand hygiene is very important to eliminate microorganisms from the skin, preventing the spread of disease, protect themselves against the aggressions of the environment. But the problem lies in poor adherence and negligence of professionals in performing this practice.

Ozone Gas has microbiocides interesting characteristics, is capable of acting on a large number of microorganisms including pathogenic and resistant microbiana not induce resistance.

Another resource available is ultrasound whose chemical and mechanical effects are caused by cavitation bubbles causing death bacteriana. Techniques using Ozonated Water, Ozone Gas and Ultrasound were applied in hand hygiene. Samples were collected from the digital palmar nail and hand dominant, seeded and incubated.

Quantitative microbiological analysis was performed, and the results showed a significant reduction of microorganisms in each technique separately. All protocols applied developed reductive action on the microbiota of hands. The technique with Ozone Gas is more efficient to decrease, followed by the combined application of Ozonated Water with Ultrasound, Ultrasound with Sterile Water and application of Ozonated Water.

Thus, it is imperative that further studies be conducted, because the protocols that used Ozone Gas and Ultrasound are very promising, since they were able to effectively reduce the number of CFUs even in the most critical regions, such as the nail. It is expected that in the future new protocol which is based on these technologies may have applicability in a hospital environment.

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